Improving the Tomato Genome by breeding with wild tomatoes [2017]

20170613_165801_zpst2ebyfmd
Solanum peruvianum (wild tomato with desert tolerance)

So i haven’t written a blog post in some time. Sorry about that. It has been very hectic this year. That’s not to say that i’m completely dead. And despite my busyness and absence i am still dabbling a little bit in the garden and plant breeding scene. I didn’t have the time, energy, or space to work on my purple Indian Corn or Teosinte this year. I barely made room for beans, peas, tomatoes, and a row of watermelon.

The beans are my special four corners native beans which include, New Mexico Red Appaloosa (aka. Gila River bean), Anasazi, Zuni Gold, Rio Zape, and maybe a few others. The Peas are a large growout of my 17-23 different varieties of genetically unique and rare pea varieties, some of which are segregating crosses that i did two seasons ago. And tough i don’t have many pictures i will post one below of a purple podded umbellatum-type (aka. crown pea) where all the pods come out in a jumble all at once. To have a purple podded one of these is new and kind of cool. I hope to have a yellow and red-podded umbellatum-type pea someday. The watermelon are the result of mine and Joseph Lofthouse’s Watermelon Landrace project. Joseph Lofthouse seems to be world famous now for his widely successful landrace seed varieties and breeding techniques.

20170618_165017_zpstwhhddhz
Purple Podded Umbellatum Crown Pea

Anyway, back to the tomatoes. The Tomatoes are a brand new project and sort of an offshoot of one of Joseph’s new landrace breeding projects as well and a few other fellow collaborators and breeders as well. It all started when Joseph was working on wanting to convert tomatoes to a landrace like many of his other successful crops. But there are a number of problems with that and domestic tomatoes in general.

The first problem is that domestic tomatoes are entirely self pollinating and don’t outcross all that much and have tiny closed-up flowers. Another problem is that domestic tomato flowers are not very attractive to pollinators. And the third major problem is that domestic tomatoes went through several genetic bottleneck selection events when they were domesticated that they have a very narrow genetic base. This narrow genetic base means that 1. Most tomatoes are subject to easily succumbing to disease and 2. that when they do outcross there is not much variation anyway. An average bad-tasting disease susceptible red tomato that crosses with another average bad-tasting disease susceptible red tomato means that in the end all you really get is more of the same.

My interest in all of this starts with the basic fact that in my climate here in Northern Colorado with my soil (mostly a dry clayish sandy soil where mostly desert plants grow), and the high altitude with intense sunlight and UV and the dry wind that wicks moisture out of the ground means that most garden varieties of anything don’t do all that well here unless intensely babied. This applies most especially to tomatoes. Even worse when it comes to Heirloom tomatoes. Sure heirloom tomatoes generally taste better, but to have a tomatoe plant produce like ONE good tomato through a whole season… That’s a MAJOR FAILURE in my book.

There are lots of tomato freaks out there that try to tell me that here in Colorado i can grow ANY tomato variety and be successful. And while that might be true if i replaces all my soil with compost or potting mix and provided massive amounts of water, and started them all early and planted them all out perfectly then yes maybe that would be true. But that’s not what i want to do, not should i have to do that. I should be able to just start a tomato plant and plant it where i want and not have to worry about it all that much and have it produce a decent harvest (whatever that happens to be). And not have to worry about disease, or growing slow, or not being adapted to my soil or the intense UV light or whatever. That’s where all this plant breeding comes in.

The goal(s)

  • To breed a superior tomato variety that does well for me (in dry N. Colorado)
  • To increase the genetic diversity in the tomato genome by using wild tomatoes
  • To create or recreate a tomato that is highly attractive to pollinators
  • To create a population of tomatoes that are highly outcrossing
  • To create a tomato that i actually think tastes good and NOT like cardboard

.

OLYMPUS DIGITAL CAMERA
wild tomato seeds. photo courtesy of Joseph Lofthouse

This project is still in it’s early stage, but it is progressing nicely. On Joseph’s end he is having huge success by using wild tomatoes bred with domestic tomatoes that have large showy flowers with exerted stigmas and have lots of pollen available that make them attractive to bumblebees. He is using mostly Solanum habrochaites but is starting to branch out to other wild tomatoes as well. Others are working on breeding tomatoes that produce a good harvest in under 100 days from being direct seeded and that have frost tolerance.

On my end i am experimenting with as many wild tomatoes that i can. I am evaluating several accessions of wild Galapagos tomatoes which so far are not doing much. The S. habrochaites also are not doing much. The ones i am having excitement from are the Solanum peruvianum which have silvery leaves and desert tolerance (in the roots) and a F1 hybrid between a domestic tomato and Solanum pennellii which has a different form of desert tolerance (in the leaves). I am excited about these genetics since they seem to be growing very well in my garden. The largest of any of my tomatoes is this F1 hybrid of S. pennellii. It is HUGE!!

20170613_165811_zpswbxpmtcc
F1 hybrid between domestic tomato and Solanum pennellii
20170618_171140_zpsrcegtafh
Flowers of an F1 hybrid between domestic tomato and Solanum pennellii
20170613_165826_zpsx7ejhhv5
F2 cross of domestic tomato and Solanum habrochaites
Advertisements

‘Wild Pueblo’ Squash Variety (2015)

7978546198_7dc5819629
Wild Pueblo Squash, Loveland Colorado 2012

I feel somewhat bad that i haven’t done much with plant breeding posts or other projects like my homemade Taffy Machine, or chemistry. I had some of those projects listed on my website. Unfortunately my website has been neglected my me and is currently down and redirects here. Perhaps by next year i can work on it again. Until then, i will try my best to diversify my blog and post some of it here.

mule08

This year i was able to grow some squash. In particular i was able to grow a few plants of my ‘Wild Pueblo’ Squash. Cucurbita maxima ‘Wild Pueblo’. Wild Pueblo is the name i have given it after both of our native pueblo ancestry. The woman who was kind enough to give me seeds was originally calling it Wild Hopi. But since i do not know if this is specifically a squash grown by the Hopi i decided to rename it. In any case it seems to be an Ancestral Puebloan variety of squash that is very old.

064_zpssexfcfof
Photo by Bobbi HolyOak, 2011, Moab Utah

In the past i never really cared that much about squash. Probably because i always had the attitude that all squash are the same. But, since I’ve been trying to find my own crops that are personal and thrive in my climate (even resorting to breeding some from scratch), I’ve decided that it would be incomplete without my own squash. In fact i think my squash are becoming my favorite thing to grow. I have a feeling that squash are going to be my favorite crop. There is just something special about seeing a squash plant growing from seed. Almost sacred and special. It’s hard to explain. Corn has a similar effect on me, but even more so with squash.

87d8451f0d11f7dfd119c84faa90fb34

Wild Pueblo was originally recovered growing in the wild of southeast Utah somewhere around the Monticello area. Close to the famous Newspaper Rock historic site. It was said to be found off the beaten track growing behind an old somewhat hidden pueblo ruin. Next to the ruin was a small stream. Growing next to the stream was a large squash plant with several large ripe squash fruit. I estimate that it is possible that it could have been growing undisturbed in that area for over 150 years! If so, this thing could have some awesome genetics. I’m doing my best to grow out the seed and preserve this variety.

7696379212_68535f4a7c
Wild Pueblo Squash, 2012
7797771746_7a64ea4cd1_z
Wild Pueblo Squash, 2012, Loveland Colorado
7978520365_b09703060a
Wild Pueblo Squash on the left. Hopi White Squash on the right.

I suspect Wild Pueblo is an older variety of squash which may be related to a landrace which may have been used to breed the variety called “lakota squash”. according to reports…

“The Lakota squash, a mid-1990’s open-pollinated introduction developed at the University of Nebraska by Dr. D. P. Coyne. Dr. Coyne experimented with crosses and selections to assure more uniform pigmentation of this beautiful squash before making it available to the trade.

Lakota squash was developed from seeds obtained by the University from Nebraska’s Fort Robinson, once a prairie Cavalry post, later an agricultural site, now a National Park. The variety it was derived from is no longer in cultivation. It had been grown by Native American peoples along the Missouri Valley for centuries before the arrival of Europeans to the continent. This indigenous squash was also cultivated by the troops stationed at Nebraska’s Forts Atkinson and Robinson, and by early Nebraska settlers.”

Edit: after talking to someone at the University of Nebraska who worked with Dr. Coyne with the original germplasm of the non-hubbard parent landrace of squash i am told the fruits were actually oblong. Still might be worth trying to grow out that accession of seed before it’s no longer viable and available… i will see if i can get some seed…

I have only grown this variety twice, but even so it has some incredible diversity which i find absolutely fascinating. In time i think i can do some great things with it, perhaps even selecting it to grow even better.

062_zpse6vcbjbl
Photo by Bobbi HolyOak, 2011, Moab Utah

This year was a very odd year to grow things. Perhaps the El Nino weather was to blame. I don’t know. All i can say is that my squash took all season to grow anything at all. I only got one plant that grew big and produced a large squash, the rest were small, but contained seeds. Next year i will try to plant more and do better.

Like i said before, it has a LOT of diverse genetics!

anasazi_ruins_mesa_verde_national_park_colorado_03

All i can say for now is that it is good to be home.